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090			_aB-16978
245	1	0	_aDiscovery of a Short-Chain Dehydrogenase from Catharanthus roseus that Produces a New Monoterpene Indole Alkaloid
490	0		_vChembiochem, Feb 9., p.doi: 10.1002/cbic.201700621, 2018
520	3		_aPlant monoterpene indole alkaloids, a large class of natural products, derive from the biosynthetic intermediate strictosidine aglycone. Strictosidine aglycone, which can exist as a variety of isomers, can be reduced to form numerous different structures. We have discovered a short-chain alcohol dehydrogenase (SDR)from plant producers of monoterpene indole alkaloids (Catharanthus roseus and Rauvolfia serpentina)that reduce strictosidine aglycone and produce an alkaloid that does not correspond to any previously reported compound. Here we report the structural characterization of this product, which we have named vitrosamine, as well as the crystal structure of the SDR. This discovery highlights the structural versatility of the strictosidine aglycone biosynthetic intermediate and expands the range of enzymatic reactions that SDRs can catalyse. This discovery further highlights how a sequence-based gene mining discovery approach in plants can reveal cryptic chemistry that would not be uncovered by classical natural product chemistry approaches.
650	1	4	_aCATHARANTHUS ROSEUS
700	1	2	_aStavrinides Ak
700	1	2	_aTatsis Ec
700	1	2	_aDang Tt
700	1	2	_aCaputi L
700	1	2	_aStevenson Cem
700	1	2	_aLawson Dm
700	1	2	_aSchneider B
700	1	2	_aSchneider B
856	4	0	_uhttps://drive.google.com/file/d/1tea3RnjUjlVOxeJV8ks1FKHaMLjacUmM/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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