000			02400nam a2200289Ia 4500
003			MX-MdCICY
005			20250625160202.0
040			_cCICY
090			_aB-17009
245	1	0	_aBiochemical events during somatic embryogenesis in Coffea arabica L.
490	0		_v3 Biotech, 8(4), p.209, 2018
520	3		_aSeveral biochemical components associated with different stages of somatic embryogenesis in coffee (Coffea arabica L.)are investigated using foliar explants. Soluble sugar, starch, free amino acids and total proteins were extracted and quantified at different stages of somatic embryogenesis, such as foliar segments (initial explants), primary calluses, embryogenic calluses, globular embryos, torpedoes, cotyledonary embryos and mature fruit zygotic embryos. Total soluble sugar levels increased sixfold at the initial stages of somatic embryogenesis induction. During this period, total soluble sugar in the cultures contained approximately 99.3 percent glucose and fructose. At 67.4 ?g/mg MS, no significant changes were observed in total sugar content during the embryo's somatic maturation and regeneration. During this stage, total soluble sugar was composed of 60 percent sucrose. After primary callus formation, starch contents increased gradually until the culture's conclusion. Total free amino acids, particularly arginine, lysine, methionine, asparagine, glutamine and histidine, revealed a higher synthesis until the formation of the primary callus, after which they remain statistically constant up to the end of the process. During the induction of calluses, a gradual increase of total proteins occurred, which, in the differentiating and maturing of somatic embryos, did not differ statistically till the formation of a cotyledonary embryo, when rates decreased 21.8 percent
650	1	4	_aBIOCHEMICAL PROFILING
650	1	4	_aLARGE-SCALE PROPAGATION
650	1	4	_aLIQUID MÉDIUM
650	1	4	_aMORPHOGENESIS
650	1	4	_aRUBIACEAE
650	1	4	_aSOMATIC EMBRYOS
700	1	2	_aBartos, P.M.C.
700	1	2	_aGomes, H.T.
700	1	2	_aDo Amaral, L.I.V.
700	1	2	_aTeixeira, J.B.
700	1	2	_aScherwinski-Pereira, J.E.
856	4	0	_uhttps://drive.google.com/file/d/1YHjBlBA14NKyMJxyR_Ng7gqKUuAXisWe/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c51173 _d51173