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245	1	0	_aPrecision genome engineering through adenine base editing in plants
490	0		_vNature Plants, 4, p.427-431, 2018
520	3		_aThe recent development of adenine base editors (ABEs)has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide 'proof of concept' for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.
650	1	4	_aGENOME ENGINEERING
700	1	2	_aKang, B. C.
700	1	2	_aYun, J. Y.
700	1	2	_aKim, S. T.
700	1	2	_aShin, Y.
700	1	2	_aRyu, J.
700	1	2	_aChoi, M.
700	1	2	_aKim, J. S.
856	4	0	_uhttps://drive.google.com/file/d/1HCKqQb1qkb_EqsQQWmrXeC5zKDGyWCth/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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