000			01297nam a2200229Ia 4500
003			MX-MdCICY
005			20250625160205.0
040			_cCICY
090			_aB-17141
245	1	0	_aCRISPR-Cas9 genome editing induces a p53-mediated DNA damage response
490	0		_vNature Medicine, 24, p.927-930, 2018
520	3		_aHere, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway. Inhibition of p53 prevents the damage response and increases the rate of homologous recombination from a donor template. These results suggest that p53 inhibition may improve the efficiency of genome editing of untransformed cells and that p53 function should be monitored when developing cell-based therapies utilizing CRISPR-Cas9.
650	1	4	_aCRISPR-CAS9
700	1	2	_aHaapaniemi, E.
700	1	2	_aBotla, S.
700	1	2	_aPersson, J.
700	1	2	_aSchmierer, B.
700	1	2	_aTaipale, J.
856	4	0	_uhttps://drive.google.com/file/d/146tLCpYdLB1fxzYd9jMdjyZHMNQKpRrH/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c51304 _d51304