000			02105nam a2200337Ia 4500
003			MX-MdCICY
005			20250625160205.0
040			_cCICY
090			_aB-17159
245	1	0	_aCytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa)and avocado (Persea americana); evidence for related enzymes with different activities
490	0		_vBiochimica et Biophysica Acta, 1201(1), p.94-100, 1994
520	3		_aA cytochrome P-450 present in ripening avocado (Persea americana)fruit mesocarp (CYTP71A1)had previously been shown to metabolize the monoterpenoids nerol and geraniol (Hallahan et al. (1992)Plant Physiol. 98, 1290-1297). Using DNA encoding CYP71A1 as a hybridization probe, we have shown by Southern analysis that a related gene is present in the catmint, Nepeta racemosa. RNA blot analysis, together with Western analysis of catmint leaf polypeptides using avocado cyt P-450 antiserum, showed that a closely related gene is expressed in catmint leaves. Cytochrome P-450 in catmint microsomes catalysed the specific hydroxylation of nerol and geraniol at C-10, whereas avocado CYP71A1, in either avocado microsomes or heterologously expressed in yeast, catalysed 2,3- or 6,7-epoxidation of these substrates. These results suggest that orthologous genes of the CYP71 family are expressed in these two plant species, but catalyse dissimilar reactions with monoterpenoid substrates.
650	1	4	_aCYTOCHROME P-450
650	1	4	_aPLANT ENZYME
650	1	4	_aSUBSTRATE SPECIFICITY
650	1	4	_aMONOTERPENOID
650	1	4	_aGENES
650	1	4	_aHYBRIDIZATION
650	1	4	_a(N. RACEMOSA)
650	1	4	_a(P. AMERICANA)
700	1	2	_aHallahan, D. L.
700	1	2	_aLau, S. M. C.
700	1	2	_aHarder, P. A.
700	1	2	_aSmiley, D. W.
700	1	2	_aDawson, G. W.
700	1	2	_aPickett, J. A.
700	1	2	_aO'Keefe, D. P.
856	4	0	_uhttps://drive.google.com/file/d/1A3M-A9XFSMREcTjDp2E0adgqHVjxQHRO/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c51322 _d51322