000 02390nam a2200277Ia 4500
003 MX-MdCICY
005 20250625160207.0
040 _cCICY
090 _aB-17274
245 1 0 _aTaxonomic characterization of a marine Nannochloropsis species, N. oceanica sp. nov.(Eustigmatophyceae)
490 0 _vPhycologia, 41(3), p.273-279, 2002
520 3 _aTen strains of Nannochloropsis species in the Marine Biotechnology Institute Culture Collection (MBIC), Japan, were classified as N. granulata (one strain), N. salina (one strain), N. gaditana (three strains)and N. oceanica Suda & Miyashita sp. nov. (five strains)by determining nearly complete 18S rDNA sequences. The 18S rDNA sequences of strains belonging to the same species were all identical. Rubisco (rbcL)gene sequences of the 10 strains were also determined. The substitution frequencies among the rbcL genes in these four species were more than three times higher than those among 18S rDNA, especially between N. gaditana and N. salina, where 16 substitutions in the rbcL genes were found, in contrast to one substitution in 18S rDNA. These data suggest strongly that rbcL gene sequences provide better resolution than 18S rDNA sequences for classification of Nannochloropsis species. Morphology was characterized by light microscopy (LM)of exponentially growing cells. Cells of N. gaditana and N. salina were cylindrical to oval. The majority of cells of other species were globose. Cells of N. granulata had many refractile granules and other granular inclusions, which exhibited vigorous Brownian movement. Few N. oculata had refractile granules. Some N. oceanica had refractile granules and granular inclusions that were nonmotile. LM observation of old cells and electron microscopy of cells of all growth stages revealed no morphological traits that distinguish between these species.
650 1 4 _aALGAE
650 1 4 _aEUKARYOTA
650 1 4 _aEUSTIGMATOPHYCEAE
650 1 4 _aMIYASHITA
650 1 4 _aNANNOCHLOROPSIS
650 1 4 _aNANNOCHLOROPSIS GADITANA
650 1 4 _aNANNOCHLOROPSIS GADITANA
700 1 2 _aSuda, S.
700 1 2 _aAtsumi, M.
700 1 2 _aMiyashita, H.
856 4 0 _uhttps://drive.google.com/file/d/1GoPUexDgg6BZlN24sXDiKRAShpVzBnzG/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 250602s9999 xx |||||s2 |||| ||und|d
999 _c51437
_d51437