000			01942nam a2200301Ia 4500
003			MX-MdCICY
005			20250625160209.0
040			_cCICY
090			_aB-17343
245	1	0	_aSpatial control of in vivo CRISPR-Cas9 genome editing via nanomagnets
490	0		_vNature Biomedical Engineering, 3(2), p.126-136, 2018
520	3		_aThe potential of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9)-based therapeutic genome editing is hampered by difficulties in the control of the in vivo activity of CRISPR-Cas9. To minimize any genotoxicity, precise activation of CRISPR-Cas9 in the target tissue is desirable. Here, we show that, by complexing magnetic nanoparticles with recombinant baculoviral vectors (MNP-BVs), CRISPR-Cas9-mediated genome editing can be activated locally in vivo via a magnetic field. The baculoviral vector was chosen for in vivo gene delivery because of its large loading capacity and ability to locally overcome systemic inactivation by the complement system. We demonstrate that a locally applied magnetic field can enhance the cellular entry of MNP-BVs, thereby avoiding baculoviral vector inactivation and causing a transient transgene expression in the target tissue. Because baculoviral vectors are inactivated elsewhere, gene delivery and in vivo genome editing via MNP-BVs are tissue specific.
650	1	4	_aGENE TRANSFER
650	1	4	_aMAGNETIC FIELDS
650	1	4	_aNANOMAGNETICS
650	1	4	_aNANOPARTICLES
650	1	4	_aTISSUE
650	1	4	_aVECTORS
700	1	2	_aZhu, H.
700	1	2	_aZhang, L.
700	1	2	_aTong, S.
700	1	2	_aLee, C. M.
700	1	2	_aDeshmukh, H.
700	1	2	_aBao, G.
856	4	0	_uhttps://drive.google.com/file/d/1_Oe_pkGzQM9Mypivy4lo8vLLavqFdKp0/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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