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003			MX-MdCICY
005			20250625160209.0
040			_cCICY
090			_aB-17346
245	1	0	_aCRISPR-Local: a local single-guide RNA (sgRNA)design tool for non-reference plant genomes
490	0		_vBioinformatics, 35(14), p.2501-2503, 2018
520	3		_aCRISPR-Local is a high-throughput local tool for designing single-guide RNAs (sgRNAs)in plants and other organisms that factors in genetic variation and is optimized to generate genome-wide sgRNAs. CRISPR-Local outperforms other sgRNA design tools in the following respects: (i)designing sgRNAs suitable for non-reference varieties; (ii)screening for sgRNAs that are capable of simultaneously targeting multiple genes; (iii)saving computational resources by avoiding repeated calculations from multiple submissions and (iv)running offline, with both command-line and graphical user interface modes and the ability to export multiple formats for further batch analysis or visualization. We have applied CRISPR-Local to 71 public plant genomes, using both CRISPR/Cas9 and CRISPR/cpf1 systems
650	1	4	_aPLANT GENOMES
700	1	2	_aSun, J.
700	1	2	_aLiu, H.
700	1	2	_aLiu, J.
700	1	2	_aCheng, S.
700	1	2	_aPeng, Y.
700	1	2	_aZhang, Q.
700	1	2	_aChen, L. L.
856	4	0	_uhttps://drive.google.com/file/d/1hxch3StGQ-bnwyQjzUULl3r0cQSUZQRI/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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