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003			MX-MdCICY
005			20250625160209.0
040			_cCICY
090			_aB-17373
245	1	0	_aGenome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM
490	0		_vNature Plants, 5(1), p.14-17, 2019
520	3		_aStreptococcus pyogenes Cas9 (SpCas9)is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1)can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
650	1	4	_aCRISPR
700	1	2	_aEndo, M.
700	1	2	_aMikami, M.
700	1	2	_aEndo, A.
700	1	2	_aKaya, H.
700	1	2	_aItoh, T.
700	1	2	_aNishimasu, H.
700	1	2	_aToki, S.
856	4	0	_uhttps://drive.google.com/file/d/1HHpkVe27XNeuVaBTw1SL-JVMZOv1C7DY/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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