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003			MX-MdCICY
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040			_cCICY
090			_aB-17387
245	1	0	_aAdvances in the Engineering of the Gene Editing Enzymes and the Genomes: Understanding and Handling the Off-Target Effects of CRISPR/Cas9
490	0		_vJournal of Biomedical Nanotechnology, 14b456-476, 2018
520	3		_aIn the past decades, significant progresses have been achieved in genetic engineering of nucleases. Among the genetically engineered nucleases, zinc finger nucleases, transcription activator-like (TAL)effector nucleases, and CRIPSPR/Cas9 system form a new field of gene editing. The gene editing efficiency or targeting effect and the off-target effect are the two major determinant factors in evaluating the usefulness of a new enzyme. Engineering strategies in improving these gene editing enzymes, particularly in minimizing their off-target effects, are the focus of this paper. Examples of using these genetically engineered enzymes in genome modification are discussed in order to better understand the requirement of engineering efforts in obtaining more powerful and useful gene editing enzymes. In addition, the identification of naturally existed anti-Cas proteins has been employed in minimizing off-target effects. Considering the future application in human gene therapy, optimization of these well recognized gene editing enzymes and exploration of more novel enzymes are both required. Before people find an ideal gene editing system having virtually no off-target effect, technologies used to screen and identify off-target effects are of importance in clinical trials employing gene therapy.
650	1	4	_aGENE EDITING
650	1	4	_aGENOME
650	1	4	_aENGINEERING
650	1	4	_aGENE THERAPY
700	1	2	_aYin Y.
700	1	2	_aWang Q
700	1	2	_aXiao L.
700	1	2	_aWang F.
700	1	2	_aSong Z.
700	1	2	_aCuilan Z.
700	1	2	_aLiu X
700	1	2	_aLiu X
700	1	2	_aHe N.
700	1	2	_aLi K.
700	1	2	_aFeng Y.
700	1	2	_aZhang J.
856	4	0	_uhttps://drive.google.com/file/d/1FKwI_yA9uM488MifOu3sovUn3hLLPlis/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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