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245	1	0	_aExtraction of bixin from annatto seeds using combined technologies
490	0		_vThe Journal of Supercritical Fluids, 100, p.,175-183, 2015
520	3		_aBixin is the most used carothenoid in food industry. It is conventionally extracted from annatto seeds (Bixa orellana L.)by alkaline solutions or organic solvents, producing bixin with low purity and generating toxic waste. This study aimed to compare different clean technologies for extraction of nutraceuticals (bixin and tocols)from annatto seeds, using a fixed bed extractor. The solvents used were supercritical CO2, ethanol and a mixture of ethanol and water. Conventional extractions using water and chloroform were also conducted, for comparison. Scanning electron microscopy was used to evaluate the surface of seeds and confirm experimental results. The best extraction efficiencies (for bixin)were using ethanol (ambient pressure)and a sequential extraction (supercritical dioxide of carbon as pretreatment followed by ethanol). This last result has also shown a good recovery of d-tocotrienol, a powerful natural antioxidant. Higher concentrations of bixin in extracts were obtained using pure ethanol at ambient and high pressures.
650	1	4	_aANNATTO
650	1	4	_aBIXIN
650	1	4	_aSUPERCRITICAL FLUID EXTRACTION
650	1	4	_aTOCOLS
700	1	2	_aTaham, T.
700	1	2	_aCabral, F. A.,
700	1	2	_aBarrozo, M. A.
856	4	0	_uhttps://drive.google.com/file/d/1Mpz6oNP_LwWmcLimOmOSlPjj1HSo3ME3/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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