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090 _aB-17414
245 1 0 _aAn enzyme from lupin seeds forming alanine derivatives of cytokinins
490 0 _vPhytoChemistry, 22(2), p. 375-381, 1983
520 3 _aA new enzyme, which catalyses the conversion of the cytokinin zeatin to the alanine conjugate lupinic acid, has been partly purified from developing lupin seed (Lupinus luteus). Paired-ion, reverse phase HPLC was adapted to analyse the enzyme reaction quantitatively. The enzyme used O-acetyl-l-serine as the source of the amino acid residue, and it interacted with substrates in a ping pong bi bi mechanism. A number of adenine derivatives served as substrates, but preference was shown for compounds with high cytokinin activity. The possible role of the enzyme, tentatively called B-(9-cytokinin)alanine synthase or lupinic acid synthase, in the regulation of hormone activity is discussed
650 1 4 _aLUPINUS LUTEUS
650 1 4 _aLEGUMINOSAE
650 1 4 _aCYTOKININ METABOLISM
650 1 4 _aENZYME FORMING ALANYL CONJUGATES
650 1 4 _aPURIFICATION
700 1 2 _aEntsch, B.
700 1 2 _aParker, C. W.
700 1 2 _aLetham, D. S.
856 4 0 _uhttps://drive.google.com/file/d/1QY244TKfvENgV9T7zbtYkGHGexcS87Ot/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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