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245	1	0	_aEvidence for Energy-Dependent 14C-Photoassimilate Retention in Isolated Tobacco Mesophyll Cells
490	0		_vPlant Physiology, 76, p.753-758, 1984
520	3		_a14C-photoassimilate release from isolated tobacco (Nicotiana tabacum L. cv Xanthi nc.)mesophyll cells was gradual and much of the photoassimilate was retained for several hours. 14C-product retention was enhanced by Ca2' and impaired by EDTA and ATP. Mg2' reduced the ATP-enhanced "C-efflux suggesting that ATP impairs product retention by modifying cell membrane permeability, possibly as a result of its chelating properties. Various metabolic inhibitors increased "4C-efflux indicating an energy requirement for "C-product retention. Profiles of "4C-product distribution in the cells and suspending medium indicated that "C-efflux from cells treated with carbonylcyanide p-trifluoromethoxyphenylhydrazone occurred by passive diffusion. Coupled with the observation that exogenous sucrose increased IC-efflux and the demonstration of C-sucrose uptake, the results suggest that product retention is partially due to the activity of an energy-dependent uptake process which recovers much of the photoassimilate lost from the cells. Under certain conditions, carbonylcyanide p-trifluoromethoxyphenylhydrazone reduced "4C-efflux. The apparently contradictory effects of the uncoupler appeared to be related to the intracellular photoassimilate concentration and may be explained by the partitioning of photoassimilate within the cells
650	1	4	_aENERGY-DEPENDENT
650	1	4	_a14C-PHOTOASSIMILATE RETENTION
650	1	4	_aISOLATED TOBACCO MESOPHYLL CELLS
700	1	2	_aScorer, Keith N.
856	4	0	_uhttps://drive.google.com/file/d/1Aunr77kiZQJshmusfM1uU7aknUJDl2I4/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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