000 01239nam a2200217Ia 4500
003 MX-MdCICY
005 20250625160217.0
040 _cCICY
090 _aB-17806
245 1 0 _aTandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes
490 0 _vCurrent Protocols in protein Science, 96, p. https://doi.org/10.1002/cpps.84, 2019
520 3 _aAffinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP)primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure.
700 1 2 _aAdelmant, G.
700 1 2 _aGarg, B. K.
700 1 2 _aTavares, M.
700 1 2 _aCard, J. D.
700 1 2 _aMarto, J. A.
856 4 0 _uhttps://drive.google.com/file/d/1LgnnvgFzFv1Kpm0LFB9kXTYFjoc6X8Ot/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 250602s9999 xx |||||s2 |||| ||und|d
999 _c51967
_d51967