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| 005 | 20250625160217.0 | ||
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| 090 | _aB-17806 | ||
| 245 | 1 | 0 | _aTandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes |
| 490 | 0 | _vCurrent Protocols in protein Science, 96, p. https://doi.org/10.1002/cpps.84, 2019 | |
| 520 | 3 | _aAffinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP)primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure. | |
| 700 | 1 | 2 | _aAdelmant, G. |
| 700 | 1 | 2 | _aGarg, B. K. |
| 700 | 1 | 2 | _aTavares, M. |
| 700 | 1 | 2 | _aCard, J. D. |
| 700 | 1 | 2 | _aMarto, J. A. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1LgnnvgFzFv1Kpm0LFB9kXTYFjoc6X8Ot/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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