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090			_aB-17808
245	1	0	_aProteome Analysis Using Gel-LC-MS/MS
490	0		_vCurrent Protocols in protein Science, 96, p. https://doi.org/10.1002/cpps.93, 2019
520	3		_aThis article describes processing of protein samples using 1D SDS gels prior to protease digestion for proteomics workflows that subsequently utilize reversed-phase nanocapillary ultra-high-pressure liquid chromatography (LC)coupled to tandem mass spectrometry (MS/MS). The resulting LC-MS/MS data are used to identify peptides and thereby infer proteins present in samples ranging from simple mixtures to very complex proteomes. Bottom-up proteome studies usually involve quantitative comparisons across several or many samples. For either situation, 1D SDS gels represent a simple, widely available technique that can be used to either fractionate complex proteomes or rapidly clean up low microgram samples with minimal losses. After gel separation and staining/destaining, appropriate gel slices are excised, and in-gel reduction, alkylation, and protease digestion are performed. Digests are then processed for LC-MS/MS analysis. Protocols are described for either sample fractionation with high-throughput processing of many samples or simple cleanup without fractionation. An optional strategy is to conduct in-solution reduction and alkylation prior to running gels, which is advantageous when a large number of samples will be separated into large numbers of fractions. Optimization of trypsin digestion parameters and comparison to in-solution protease digestion are also described.
700	1	2	_aGoldman, A. R.
700	1	2	_aBeer, L. A.
700	1	2	_aTang, H. Y.
700	1	2	_aHembach, P.
700	1	2	_aZayas-Bazan, D.
700	1	2	_aSpeicher, D. W.
856	4	0	_uhttps://drive.google.com/file/d/1IqOjZ90eMUoQNK_q7FqiP1hTxwiLISXl/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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