000			01398nam a2200217Ia 4500
003			MX-MdCICY
005			20250625160218.0
040			_cCICY
090			_aB-17819
245	1	0	_aNucleic Acid Detection of Plant Genes Using CRISPR-Cas13.
490	0		_vThe CRISPR Journal,, 2(3), p.165-171, 2019
520	3		_aNucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identi?cation. Here, we use a modi?ed version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (*15min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This ?eld-ready SHERLOCK platform with color-based lateral ?ow readout can be applied for detection and quantitation of genes in a range of agricultural applications.
650	1	4	_aCRISPR-CAS13
700	1	2	_aAbudayyeh, O.O.
700	1	2	_aGootenberg, J.S.
700	1	2	_aKellner, M.J.
700	1	2	_aZhang,F.
856	4	0	_uhttps://drive.google.com/file/d/1EWsIVkxerCapNNNXM0Qwbo45qPldg0eb/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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