000			02598nam a2200253Ia 4500
003			MX-MdCICY
005			20250625160218.0
040			_cCICY
090			_aB-17854
245	1	0	_aDeletion of the Non-Ribosomal Peptide Synthetase Gene nps1 in the Fungus Clonostachys rosea Attenuates Antagonism and Biocontrol of Plant Pathogenic Fusarium and Nematodes
490	0		_vPhytoPathology, https://doi.org/10.1094/PHYTO-02-19-0042-R, 2019
520	3		_aSecondary metabolites produced by biological control agents may influence the outcome of their interactions with plant pathogenic microorganisms and plants. In the present study, we investigated the role of the nonribosomal peptide synthetase gene nps1 expressed by the biocontrol fungus Clonostachys rosea. A gene expression analysis showed that nps1 was induced during confrontations with the plant pathogenic fungus Botrytis cinerea. Gene deletion strains of nps1 displayed increased growth rates and conidiation. However, the nematicidal activity of culture filtrates from C. rosea ?nps1 strains was significantly weaker than that from wild-type filtrates (P ? 0.001); after 24 h of incubation with culture filtrates from nps1 deletion strains, only 13 to 33 percent of a mixed community of nematodes were dead compared with 42 percent of nematodes incubated with wild-type culture filtrates. The ?nps1 strains also showed reduced biocontrol efficacy during pot experiments, thus failing to protect wheat seedlings from foot rot disease caused by the plant pathogenic fungus Fusarium graminearum. Furthermore, C. rosea ?nps1 strains were not able to reduce populations of plant-parasitic nematodes in soil or in roots of wheat as efficiently as the wild-type strain. Both C. rosea wild-type and ?nps1 strains increased the dry shoot weight and shoot length of wheat by 20 and 13 percent, respectively. We showed that NPS1, a putative nonribosomal peptide synthetase encoded by nps1, is a biocontrol factor, presumably by producing a hitherto unknown nonribosomal peptide compound with antifungal and nematicidal properties that contributes to the biocontrol properties of C. rosea.
650	1	4	_aFUNGUS
650	1	4	_aCLONOSTACHYS ROSEA
700	1	2	_aIqbal, M.
700	1	2	_aDubey, M.
700	1	2	_aBroberg, A.
700	1	2	_aViketoft, M.
700	1	2	_aFunck Jensen, D.
700	1	2	_aKarlsson, M.
856	4	0	_uhttps://drive.google.com/file/d/1tpGEc0aaKAtJK5G4Ay3lI8UuA-PbWRVB/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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999			_c52015 _d52015