000			02032nam a2200289Ia 4500
003			MX-MdCICY
005			20250625160218.0
040			_cCICY
090			_aB-17856
245	1	0	_aSirtuin 7 promotes 45S pre-rRNA cleavage at site 2 and determines the processing pathway
490	0		_vJournal of cell Science, 132(17), p.jcs228601, 2019
520	3		_aIn humans, ribosome biogenesis mainly occurs in nucleoli following two alternative pre-rRNA processing pathways differing in the order in which cleavages take place but not by the sites of cleavage. To uncover the role of the nucleolar NAD+-dependent deacetylase sirtuin 7 in the synthesis of ribosomal subunits, pre-rRNA processing was analyzed after sirtinol-mediated inhibition of sirtuin 7 activity or depletion of sirtuin 7 protein. We thus reveal that sirtuin 7 activity is a critical regulator of processing of 45S, 32S and 30S pre-rRNAs. Sirtuin 7 protein is primarily essential to 45S pre-rRNA cleavage at site 2, which is the first step of processing pathway 2. Furthermore, we demonstrate that sirtuin 7 physically interacts with Nop56 and the GAR domain of fibrillarin, and propose that this could interfere with fibrillarin-dependent cleavage. Sirtuin 7 depletion results in the accumulation of 5' extended forms of 32S pre-rRNA, and also influences the localization of fibrillarin. Thus, we establish a close relationship between sirtuin 7 and fibrillarin, which might determine the processing pathway used for ribosome biogenesis.
650	1	4	_aPRE-RRNA PROCESSING
650	1	4	_aRIBOSOME
650	1	4	_aSIRTINOL
650	1	4	_aSIRT7
650	1	4	_aNUCLEOLUS
650	1	4	_aDEACETYLASE
700	1	2	_aSirri, V.
700	1	2	_aGrob, A.
700	1	2	_aBerthelet, J.
700	1	2	_aJourdan, N.
700	1	2	_aRoussel, P.
856	4	0	_uhttps://drive.google.com/file/d/19XtNBDhigVRs05pV8V8D8AaY71WQjqwN/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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