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090			_aB-17898
245	1	0	_aNascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.
490	0		_vMolecular Cell, https://doi.org/10.1016/j.molcel.2019.08.014, 2019
520	3		_aFibrillar centers (FCs)and dense fibrillar components (DFCs)are essential morphologically distinct sub-regions of mammalian cell nucleoli for rDNA transcription and pre-rRNA processing. Here, we report that a human nucleolus consists of several dozen FC/DFC units, each containing 2-3 transcriptionally active rDNAs at the FC/DFC border. Pre-rRNA processing factors, such as fibrillarin (FBL), form 18-24 clusters that further assemble into the DFC surrounding the FC. Mechanistically, the 5? end of nascent 47S pre-rRNA binds co-transcriptionally to the RNA-binding domain of FBL. FBL diffuses to the DFC, where local self-association via its glycine- and arginine-rich (GAR)domain forms phase-separated clusters to immobilize FBL-interacting pre-rRNA, thus promoting directional traffic of nascent pre-rRNA while facilitating pre-rRNA processing and DFC formation. These results unveil FC/DFC ultrastructures in nucleoli and suggest a conceptual framework for considering nascent RNA sorting using multivalent interactions of their binding proteins.
650	1	4	_aFIBRILLARIN
650	1	4	_aGLYCINE- AND ARGININE-RICH
650	1	4	_aINTRINSICALLY DISORDERED REGION
650	1	4	_aRNA BINDING DOMAIN
650	1	4	_aRNA SORTING
650	1	4	_aNUCLEOLUS
650	1	4	_aCONDENSATES
650	1	4	_aRIBOSOMAL RNA
650	1	4	_aPHASE SEPARATION
650	1	4	_aSUPER-RESOLUTION MICROSCOPY
700	1	2	_aYao, R. W.
700	1	2	_aXu, G.
700	1	2	_aWang, Y.
700	1	2	_aShan, L.
700	1	2	_aLuan, P. F.
700	1	2	_aWang, Y.
700	1	2	_aChen, L. L.
856	4	0	_uhttps://drive.google.com/file/d/1kU9zWp_5Hl2xQ2ls5p-A9FZjotD9o3D2/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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