000			02239nam a2200265Ia 4500
003			MX-MdCICY
005			20250625160223.0
040			_cCICY
090			_aB-18087
245	1	0	_aFunctional divergence of PIN1 paralogous genes in rice.
490	0		_vPlant & Cell Physiology, 60(12), p.2720-2732, 2019
520	3		_aAuxin is a phytohormone that plays an important role in plant growth and development by forming local concentration gradients. The regulation of auxin levels is determined by the activity of auxin efflux carrier protein PIN-formed (PIN). In Arabidopsis thaliana, PIN-formed1 (PIN1)functions in inflorescence and root development. In rice (Oryza sativa L.), there are four PIN1 homologs (OsPIN1a-1d), but their functions remain largely unexplored. Hence, in this study, we created mutant alleles of PIN1 gene-pin1a, pin1b, pin1c, pin1d, pin1a pin1b and pin1c pin1d- using CRISPR/Cas9 technology and used them to study the functions of the four OsPIN1 paralogs in rice. In wild-type rice, all four OsPIN1 genes were relatively highly expressed in the root than in other tissues. Compared with the wild type, the OsPIN1 single mutants had no dramatic phenotypes, but the pin1a pin1b double mutant had shorter shoots and primary roots, fewer crown roots, reduced root gravitropism, longer root hairs and larger panicle branch angle. Furthermore, the pin1c pin1d double mutant showed no observable phenotype at the seedling stage, but showed naked, pin-shape inflorescence at flowering. These data suggest that OsPIN1a and OsPIN1b are involved in root, shoot and inflorescence development in rice, whereas OsPIN1c and OsPIN1d mainly function in panicle formation. Our study provides basic knowledge that will facilitate the study of auxin transport and signaling in rice.
650	1	4	_aCRISPR
650	1	4	_aADAPTATION
650	1	4	_aCRISPR-CAS
700	1	2	_aLi, Y.
700	1	2	_aZhu, J.
700	1	2	_aWu, L.
700	1	2	_aShao, Y.
700	1	2	_aWu, Y.
700	1	2	_aMao, C.
856	4	0	_uhttps://drive.google.com/file/d/1YHlh_ayQIuw9GfEYsG311NmX5m5pzOTV/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c52245 _d52245