000			01829nam a2200325Ia 4500
003			MX-MdCICY
005			20250625160223.0
040			_cCICY
090			_aB-18121
245	1	0	_aNucleotide sequence and expression of the glucoamylase-encoding gene (glaA)from Aspergillus oryzae.
490	0		_vGene, 108(1), p.145-150, 1991
520	3		_aThe glucoamylase-encoding gene (glaA)from Aspergillus oryzae was cloned using its cDNA as a probe, which had been isolated previously. From comparison of nucleotide (nt)sequences of genomic clones with its cDNA, the glaA gene was found to contain four short putative introns, 45-56 nt in length. The A. oryzaeglaA gene shared 62 per cent homology at the nt level with the A. nigerglaA gene with the four introns located at the same position. The 5'-flanking region contained a TATA box at nt ?72 from the start codon, and two putative CAAT sequences at nt ?87 and ?331. Genomic Southern analysis and physical mapping showed that the glaA gene is located on the smallest chromosome (3.4 Mb)of six separated bands of chromosomes. Clones containing the glaA gene, when re-introduced intro A. oryzae, resulted in a three- to eightfold increase in glucoamylase activity.
650	1	4	_aRECOMBINANT DNA
650	1	4	_aINTRON
650	1	4	_aTRANSFORMATION
650	1	4	_aGENE STRUCTURE
650	1	4	_aPHYSICAL MAPPING
650	1	4	_aSAKE
650	1	4	_aASCOMYCETES
700	1	2	_aYoji, H.
700	1	2	_aKozo, T.
700	1	2	_aKatsuhiko, K.
700	1	2	_aKatsuya, G.
700	1	2	_aChieko, K.
700	1	2	_aGakuzo, T.
700	1	2	_aShodo, H.
856	4	0	_uhttps://drive.google.com/file/d/1Hq0qf0V1mZ_uE-i080E0Kp4hYoyLSavO/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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