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090 _aB-18181
245 1 0 _aEvolution and molecular mechanism of CRISPR/Cas9 systems.
490 0 _vIn Genome Engineering via CRISPR-Cas9 System. Academic Press., p.15-25, 2020
520 3 _aCRISPR/Cas module is an RNA-guided adaptive immune system, provides sequence-specific immunity against phages and (conjugative)plasmids. CRISPR/Cas system is basically categorized into three types: (type I, II and III), on the basis of effector complexes. CRISPR/Cas9 system is repercussion of evolutionary everlasting arm race between bacteria and phages. Molecular mechanism underlying the CRISPR system entails three distinct mechanistic stages: spacer acquisition, crRNA biogenesis and interference. CRISPR/Cas9 system composed of a combination of Cas9 (an endonuclease)and a customizable single guide RNA (sgRNA), the later directs the Cas9 for recognition and degradation of invading DNA. Furthermore, Cas9 and their ilk proteins with unique properties have opened a plethora of options including DNA/RNA genome targeting, transcription/translation regulation and empower researchers to edit genes with highly precision and speed.
650 1 4 _aBACTERIA
650 1 4 _aCASCADE
650 1 4 _aCRISPR/CAS SYSTEMS
650 1 4 _aDCAS9
650 1 4 _aGENOME EDITING
650 1 4 _aRNA
700 1 2 _aBhushan, K.
856 4 0 _uhttps://drive.google.com/file/d/1OYdwUhuslep28rATaBWT__U21w7iUh83/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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