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245 1 0 _aThe use of a non-cultured autologous cell suspension and Integra® dermal regeneration template to repair full-thickness skin wounds in a porcine model: a one-step process.
490 0 _vBurns, 33(6), p.693-700, 2007
520 3 _aIntegra® is a skin substitute used for dermal reconstruction. Current clinical practice consists of two procedures, first applying Integra® to the wound and then replacing the silicone pseudo-epidermis with an epidermal autograft 3 weeks later. This two-step repair limits the clinical use of the product. An effective one-step procedure could reduce the time taken to repair and decrease the number of procedures for use of Integra®. This study examined the effects of simultaneous application of a non-cultured autologous suspension of cells, isolated using the ReCell® autologous cell harvesting device, in combination with Integra®, to achieve a one-step skin repair. In two female Yorkshire swine, 10 full-thickness wounds were created. Wounds were treated with Integra® seeded with cell suspension and compared to controls of Integra® alone and cell suspension alone. Weekly macroscopic and histological assessment demonstrated that the wounds treated simultaneously with Integra® and non-cultured autologous cells had enhanced epithelialization at an early time-point compared to controls. Wounds treated simultaneously with Integra® and cell suspension demonstrate that cells remain viable, migrate through the Integra® template and self-organize into differentiated epidermis. The results indicate that combining Integra® with autologous cells facilitates one-step skin reconstruction of a full-thickness skin wound.
650 1 4 _aBURN INJURY
650 1 4 _aDERMAL REGENERATION TEMPLATE
650 1 4 _aNON-CULTURED AUTOLOGOUS CELL SUSPENSION
700 1 2 _aWood, F. M.
700 1 2 _aStoner, M. L.
700 1 2 _aFowler, B. V.
700 1 2 _aFear, M. W.
856 4 0 _uhttps://drive.google.com/file/d/1-u4i7GX-EgEoY6Uwvpms9oAZ6E0ZhBgD/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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