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003			MX-MdCICY
005			20250625160226.0
040			_cCICY
090			_aB-18265
245	1	0	_aPrime genome editing in rice and wheat.
490	0		_vNature Biotechnology, 38(5), p.582-585, 2020
520	3		_aPrime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8 percent.
650	1	4	_aGENOME
700	1	2	_aLin, Q.
700	1	2	_aZong, Y.
700	1	2	_aXue, C.
700	1	2	_aWang, S.
700	1	2	_aJin, S.
700	1	2	_aZhu, Z.
700	1	2	_aLiu, D. R.
856	4	0	_uhttps://drive.google.com/file/d/1msMbIAuX2y4DPctUAUT28VMardk_1cbm/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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