000			02605nam a2200265Ia 4500
003			MX-MdCICY
005			20250625162413.0
040			_cCICY
090			_aB-18477
245	1	0	_aIsoflavone levels, nodulation and gene expression profiles of a CRISPR/Cas9 deletion mutant in the isoflavone synthase gene of red clover.
490	0		_vPlant Cell Reports, p.1-12., 2021
520	3		_aRed clover (Trifolium pratense)is a high-quality forage legume, well suited for grazing and hay production in the temperate regions of the world. Like many legumes, red clover produces a number of phenylpropanoid compounds including anthocyanidins, flavan-3-ols, flavanols, flavanones, flavones, and isoflavones. The study of isoflavone biosynthesis and accumulation in legumes has come into the forefront of biomedical and agricultural research due to potential for medicinal, antimicrobial, and environmental implications. CRISPR/Cas9 was used to knock out the function of a key enzyme in the biosynthesis of isoflavones, isoflavone synthase (IFS1). A hemizygous plant carrying a 9-bp deletion in the IFS1 gene was recovered and was intercrossed to obtain homozygous mutant plants. Levels of the isoflavones formononetin, biochanin A and genistein were significantly reduced in the mutant plants. Wild-type and mutant plants were inoculated with rhizobia to test the effect of the mutation on nodulation, but no significant differences were observed, suggesting that these isoflavones do not play important roles in nodulation. Gene expression profiling revealed an increase in expression of the upstream genes producing the precursors for IFS1, namely, phenylalanine ammonium lyase and chalcone synthase, but there were no significant differences in IFS1 gene expression or in the downstream genes in the production of specific isoflavones. Higher expression in genes involved in ethylene response was observed in the mutant plants. This response is normally associated with biotic stress, suggesting that the plants may have been responding to cues in the surrounding rhizosphere due to lower levels of isoflavones.
650	1	4	_aISOFLAVONE LEVELS
650	1	4	_aISOFLAVONE SYNTHASE
700	1	2	_aDinkins, R. D.
700	1	2	_aHancock, J.
700	1	2	_aCoe, B. L.
700	1	2	_aMay, J. B.
700	1	2	_aGoodman, J. P.
700	1	2	_aBass, W. T.
700	1	2	_aZhu, H.
856	4	0	_uhttps://drive.google.com/file/d/15oTWuOLSYlaQxUsadrKIT78y-RSTyQZ0/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c52628 _d52628