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090 _aB-18524
245 1 0 _aMolecular evolution and expression analysis of ADP-ribosylation factors (ARFs)from longan embryogenic callus.
490 0 _vGene, p.145461, 2021
520 3 _aADP-ribosylation modification considered as a model to study histone post-translational modification in chromatin modification. Despite it was reported in many plants, the study of ARFs gene family in longan was still unclear. In this study, 14 longan ARFs genes were identified using the longan genome (the third-generation genome)and further divided into two major groups, including the DlARF in the I-II group and the ARF-like (DlARL)in the III-V group, according to their structure and evolutionary characteristics. Whole-genome duplication (WGD)and segmental duplication events played a major role in the expansion of the DlARFs gene family, the synteny and phylogenetic analyses provided a deeper insight into the evolutionary characteristics of the DlARFs. Protein-protein interactions suggested that some DlARFs proteins may interact to participate in biological processes. Promoter analysis showed more stress response elements in DlARF5, DlGB1, DlARL1, DlARL2, and DlARL8a, suggesting that they may participate in abiotic stress. Expression profiles of DlARFs by quantitative real-time PCR (qRT-PCR)showed that they were abundant accumulation during early somatic embryogenesis (SE). Expression pattern analysis of RNA-seq and qRT-PCR revealed that some ARFs members regulated early SE, and respond to exogenous hormones and abiotic stress such as abscisic acid (ABA), gibberellin A3 (GA3), salicylic acid (SA), methyl jasmonate (MeJA), cold, and heat. Our study provides new insights for further research on the potential function of DlARFs, which may be useful for the improvement of longan.
650 1 4 _aLONGAN
650 1 4 _aADP-RIBOSYLATION FACTORS
650 1 4 _aABIOTIC STRESS
650 1 4 _aSOMATIC EMBRYOGENESIS
650 1 4 _aQUANTITATIVE ANALYSIS
700 1 2 _aShen, X.
700 1 2 _aChen, R.
700 1 2 _aChen, X.
700 1 2 _aMunir, N.
700 1 2 _aZhang, S.
700 1 2 _aXu, X.
700 1 2 _aLai, Z.
856 4 0 _uhttps://drive.google.com/file/d/1FbSodpAhmS2JCAy4L-syDrzp5qVTlwmK/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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