000			01831nam a2200301Ia 4500
003			MX-MdCICY
005			20250625162414.0
040			_cCICY
090			_aB-18550
245	1	0	_aProduction of a ruminal bacterial phytase in the green microalga Chlamydomonas reinhardtii with potential applications in monogastric animal feed
490	0		_vBioresource Technology Reports, p.100660, 2021
520	3		_aPhytases are phosphatases employed in monogastric animal feed to improve animal growth rates and protect the environment by reducing phosphorous in manure. In this study, the highly active phytase PhyAsr of the protein tyrosine phosphatase class, which was derived from the ruminal bacterium Selenomonas ruminantium, was successfully produced for the first time in the chloroplast of the green microalga Chlamydomonas reinhardtii. Two homoplasmic lines (C1 and C3)exhibited the highest level of PhyAsr expression, while the highest phytase activity was detected in the C1 line at 34 U/g dry weight. The results of this study indicate that C. reinhardtii is a suitable host for producing the phytase PhyAsr, which may be utilized as a supplement in the monogastric animal diet.
650	1	4	_aPHYTASE
650	1	4	_aMICROALGA
650	1	4	_aCHLAMYDOMONAS REINHARDTII
650	1	4	_aCHLOROPLAST
650	1	4	_aRECOMBINANT PROTEIN
700	1	2	_aPeraza-Echeverria, S.
700	1	2	_aBernardo-Candelero, S.
700	1	2	_aBaas-Espinola, F. M.
700	1	2	_aPuch-Hau, C
700	1	2	_aRivera-Solís, R. A.
700	1	2	_aEchevarría-Machado, I.
700	1	2	_aHerrera-Valencia, V. A.
856	4	0	_uhttps://drive.google.com/file/d/1oySya9AMANDjQReJhwGhGmZFyh-dG_W2/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c52700 _d52700