| 000 | 02450nam a2200289Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162414.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-18555 | ||
| 245 | 1 | 0 | _aTranscriptome-based metabolic profiling of flavonoids in Agave lechuguilla waste biomass. |
| 490 | 0 | _vPlant Science , 305, p.110748., 2021 | |
| 520 | 3 | _aAgave lechuguilla is one of the most abundant species in arid and semiarid regions of Mexico, and is used to extract fiber. However, 85 percen of the harvested plant material is discarded. Previous bioprospecting studies of the waste biomass suggest the presence of bioactive compounds, although the extraction process limited metabolite characterization. This work achieved flavonoid profiling of A. lechuguilla in both processed and non-processed leaf tissues using transcriptomic analysis. Functional annotation of the first de novo transcriptome of A. lechuguilla (255.7 Mbp)allowed identifying genes coding for 33 enzymes and 8 transcription factors involved in flavonoid biosynthesis. The flavonoid metabolic pathway was mostly elucidated by HPLC-MS/MS screening of alcoholic extracts. Key genes of flavonoid synthesis were higher expressed in processed leaf tissues than in non-processed leaves, suggesting a high content of flavonoids and glycoside derivatives in the waste biomass. Targeted HPLC-UV-MS analyses confirmed the concentration of isorhamnetin (1251.96 µg), flavanone (291.51 µg), hesperidin (34.23 µg), delphinidin (24.23 µg), quercetin (15.57 µg), kaempferol (13.71 µg), cyanidin (12.32 µg), apigenin (9.70 µg)and catechin (7.91 µg)per gram of dry residue. Transcriptomic and biochemical profiling concur in the potential of lechuguilla by-products with a wide range of applications in agriculture, feed, food, cosmetics, and pharmaceutical industries. | |
| 650 | 1 | 4 | _aAGAVE LECHUGUILLA |
| 650 | 1 | 4 | _aWASTE BIOMASS |
| 650 | 1 | 4 | _aFLAVONOIDS BIOSYNTHESIS |
| 650 | 1 | 4 | _aTRANSCRIPTOMIC ANALYSIS |
| 650 | 1 | 4 | _aMETABOLITE PROFILING |
| 650 | 1 | 4 | _aHPLC ANALYSIS |
| 700 | 1 | 2 | _aMorreeuw, Z. P. |
| 700 | 1 | 2 | _aEscobedo-Fregoso, C. |
| 700 | 1 | 2 | _aRíos-González, L. J. |
| 700 | 1 | 2 | _aCastillo-Quiroz, D. |
| 700 | 1 | 2 | _aReyes, A. G. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/15y28_0VVCpX3Yobc_5wXhCAfSBjV0kXq/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 |
_2Loc _cREF1 |
||
| 008 | 250602s9999 xx |||||s2 |||| ||und|d | ||
| 999 |
_c52705 _d52705 |
||