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090			_aB-18584
245	1	0	_aHeterologous expression systems for plant defensin expression: examples of success and pitfalls.
490	0		_vCurrent Protein and Peptide Science, 18(4), p.391-399, 2017
520	3		_aDefensins are a superfamily of antimicrobial peptides, present in vertebrates, invertebrates, fungi and plants, suggesting that they appeared prior to the divergence in eukaryotes. The destitution of toxicity to mammalian cells of plant defensins has led to a new research ground, i.e., their potential medical use against human infectious diseases. Isolating defensins from natural sources, like plant tissues, can be time-consuming, labor intensive and usually present low yields. Strategies for large-scale production of purified active defensins have been employed using heterologous expression systems (HES)for defensin production, usually based in E. coli system. Like any other technology, HES present limitations and drawbacks demanding a careful experimental design prior the system selection. This review is proposed to discuss some of the major concerns when choosing to heterologously express plant defensins, with special attention on bacterial expression systems.
650	1	4	_aE. COLI
650	1	4	_aANTIMICROBIAL PEPTIDES
650	1	4	_aCARRIER PROTEIN
650	1	4	_aFUSION
650	1	4	_aINCLUSION BODIES
650	1	4	_aSECRETION SIGNAL
700	1	2	_aSaldanha Gazzaneo,L. R.
700	1	2	_aPandolfi, V.
700	1	2	_aLuiz Santos De Jesus,
700	1	2	_aCrovella, S.
700	1	2	_aMaria Benko-Iseppon,
700	1	2	_aDe Freitas, A. Carlos
856	4	0	_uhttps://drive.google.com/file/d/1ASBhWHh9pHSEAQ4a7gEKGwrHVANXb1YF/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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