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090			_aB-18612
245	1	0	_aEnzymatic assembly of DNA molecules up to several hundred kilobases.
490	0		_vNature methods, 6(5), p.343-345, 2009
520	3		_aWe describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5? exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool.
650	1	4	_aDNA MOLECULES
700	1	2	_aGibson, D. G.
700	1	2	_aYoung, L.
700	1	2	_aChuang, R. Y.
700	1	2	_aVenter, J. C.
700	1	2	_aHutchison, C. A.
700	1	2	_aSmith, H. O.
856	4	0	_uhttps://drive.google.com/file/d/12K9Z54da4GzV2cDBdQcdGZN4h0CAxgM2/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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