| 000 | 02291nam a2200289Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162416.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-18644 | ||
| 245 | 1 | 0 | _aDown-regulation of the E2 subunit of 2-oxoglutarate dehydrogenase modulates plant growth by impacting carbon-nitrogen metabolism in Arabidopsis thaliana. |
| 490 | 0 | _vPlant & Cell Physiology, https://doi.org/10.1093/pcp/pcab036, 2021 | |
| 520 | 3 | _aIn Arabidopsis thaliana, two genes encode the E2 subunit of the 2-oxoglutarate dehydrogenase (2-OGDH), a multimeric complex composed of three subunits. To functionally characterize the isoforms of E2 subunit, we isolated Arabidopsis mutant lines for each gene encoding the E2 subunit and performed a detailed molecular and physiological characterization of the plants under controlled growth conditions. The functional lack of expression of E2 subunit isoforms of 2-OGDH increased plant growth, reduced dark respiration, and altered carbohydrate metabolism without changes in the photosynthetic rate. Interestingly, plants from e2-ogdh lines also exhibited reduced seed weight without alterations in total seed number. We additionally observed that downregulation of 2-OGDH activity led to minor changes in the levels of tricarboxylic acid (TCA)cycle intermediates without clear correlation with the reduced expression of specific E2-OGDH isoforms. Furthermore, the e2-ogdh mutant lines exhibited a reduction by up to 25 percent in the leaf total amino acids without consistent changes in the amino acid profile. Taken together, our results indicate that the two isoforms of E2 subunit play a similar role in carbon-nitrogen metabolism, in plant growth and in seed weight. | |
| 650 | 1 | 4 | _aPRIMARY METABOLISM |
| 650 | 1 | 4 | _a2-OGDH |
| 650 | 1 | 4 | _aRESPIRATION |
| 650 | 1 | 4 | _aTCA CYCLE |
| 700 | 1 | 2 | _aCondori-Apfata, J. A. |
| 700 | 1 | 2 | _aBatista-Silva, W. |
| 700 | 1 | 2 | _aMedeiros, D. B. |
| 700 | 1 | 2 | _aRafael Vargas, J. |
| 700 | 1 | 2 | _aValente, L. M. L. |
| 700 | 1 | 2 | _aLuis Perez Diaz, J. |
| 700 | 1 | 2 | _aNunes-Nesi, A. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1WZDG1feie7irQ1bO8NuIrD7BO_GKmYdF/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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