000			01847nam a2200277Ia 4500
003			MX-MdCICY
005			20250625162416.0
040			_cCICY
090			_aB-18655
245	1	0	_aApplication of CRISPR/Cas System in the Metabolic Engineering of Small Molecules.
490	0		_vMolecular Biotechnology, 63, p.459-476, 2021
520	3		_aClustered regularly interspaced short palindromic repeats (CRISPR)and their associated Cas protein technology area is rapidly growing technique for genome editing and modulation of transcription of several microbes. Successful engineering in microbes requires an emphasis on the aspect of efficiency and targeted aiming, which can be employed using CRISPR/Cas system. Hence, this type of system is used to modify the genome of several microbes such as yeast and bacteria. In recent years, CRISPR/Cas systems have been chosen for metabolic engineering in microbes due to their specificity, orthogonality, and efficacy. Therefore, we need to review the scheme which was acquired for the execution of the CRISPR/Cas system for the modification and metabolic engineering in yeast and bacteria. In this review, we highlighted the application of the CRISPR/Cas system which has been used for the production of small molecules in the microbial system that is chemically and biologically important.
650	1	4	_aCRISPR-CAS
650	1	4	_aMETABOLIC ENGINEERING
650	1	4	_aSMALL MOLECULES
700	1	2	_aSingh, R.
700	1	2	_aChandel, S.
700	1	2	_aGhosh, A.
700	1	2	_aDey, D.
700	1	2	_aChakravarti, R.
700	1	2	_aRoy, S.
700	1	2	_aGhosh, D.
856	4	0	_uhttps://drive.google.com/file/d/1dMp7CiUwJfVS6r4coi9LUcJZwa4IeHqk/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c52801 _d52801