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090			_aB-18742
245	1	0	_aPloidy level, epigenetic and in vitro environment infuence the indirect somatic embryogenesis of the new synthetic autoallohexaploid Cofea
490	0		_vPlant Cell, Tissue and Organ Culture, p.1-11, 2021
520	3		_aAutoallohexaploid plantlets of "Híbrido de Timor" (HT, Cofea)were recently regenerated by chromosome set doubling from allotriploid HT. Besides the in vitro propagation and chromosome doubling set, indirect somatic embryogenesis allows us to investigate the short-term polyploidy consequences. We aimed to establish and compare the indirect somatic embryogenesis in natural allotriploid and synthetic autoallohexaploid HT, evaluating the role of the in vitro environment, ploidy level and global level of 5-methylcytosine on somatic embryo regeneration and karyotype stability. Our results showed that the autoallohexaploid needed more time for induction and proliferation of friable calli, showing lower mean numbers of responsive explants and a higher mean level of 5-methylcytosine. Autoallohexaploid also showed a higher 5-methylcytosine level during the somatic embryo regeneration, but both hybrids exhibited the same mean value of mature cotyledonary somatic embryos for friable callus. Regarding the in vitro environment, the activated charcoal at 8 and 16 g L-1 increased global level of 5-methylcytosine and the mean number of abnormal somatic embryo in autoallohexaploid. Based on these results, the autoallohexaploid exhibited a lower in vitro response compared to its allotriploid ancestor, despite having at least twice as many copies of in vitro response genes. Besides, our data evidenced that the in vitro environment, ploidy level and global 5-methylcytosine level infuence the Cofeaindirect somatic embryogenesis, showing the outcome of the short-term induced polyploidy. Therefore, we showed the euploidy infuence during indirect somatic embryogenesis, demonstrating the relevance of the "omics" evaluations of the new Cofeagermplasm in relation to their ancestor.
650	1	4	_aCOFEE
650	1	4	_aIN VITRO PROPAGATION
650	1	4	_aPOLYPLOIDY
650	1	4	_aPLANT TISSUE CULTURE
650	1	4	_aEPIGENETIC
700	1	2	_aMorais Oliveira, João Paulo De
700	1	2	_aArruda Sanglard, Natália
700	1	2	_aFerreira, Adésio
700	1	2	_aRonildo Clarindo, Wellington
856	4	0	_uhttps://drive.google.com/file/d/13yzGW2mtdePaks839xVHeEmOHMcxribk/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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