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090 _aB-18745
245 1 0 _aIn vitro regeneration of Phaseolus vulgaris L. via direct and indirect organogenesis
490 0 _vPlant Biotechnology Reports, 15, p.279-288, 2021
520 3 _aA stable and efcient regeneration system is a necessary condition for the genetic transformation of Phaseolus vulgarisL. In this study, cotyledonary nodes with one cotyledon, cotyledonary nodes with two cotyledons, embryonic axes and root segments of 5-day-old germinated seeds were used as explants to evaluate explants to induce shoots and calli. Besides, using cotyledonary nodes with one cotyledon as explants, the efects of diferent concentrations of auxins and cytokinins on the direct and indirect organogenesis of common bean were investigated. As a result, embryonic axes and cotyledonary nodes with two cotyledons were the most efective explant types for the formation of shoots and calli. The cotyledonary nodes with one cotyledon were cultured in the shoot induction medium containing 7 mg L-16-benzyladenine (BAP)and 0.2 mg L -1a-naphthaleneacetic acid (NAA). 74.21
650 1 4 _aDIRECT ORGANOGENESIS
650 1 4 _aINDIRECT ORGANOGENESIS
650 1 4 _aPHASEOLUS VULGARISL.
650 1 4 _aREGENERATION
700 1 2 _aYu, Yan
700 1 2 _aLiu, Dajun
700 1 2 _aLiu, Chang
700 1 2 _aYan, Zhishan
700 1 2 _aYang, Xiaoxu
700 1 2 _aFeng, Guojun
856 4 0 _uhttps://drive.google.com/file/d/1iLKNqogar-pdT_EaP6IBfJTsHhbpq2eK/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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