000			02162nam a2200265Ia 4500
003			MX-MdCICY
005			20250625162419.0
040			_cCICY
090			_aB-18790
245	1	0	_aGenome-wide discovery of DNA variants in cucumber somaclonal lines
490	0		_vGene, 736, p.14441, 2020
520	3		_aThe emergence of somaclonal variability in in vitro cultures is undesirable during micropropagation, but this phenomenon may be a source of genetic variability sought by breeders. The main factors that affect the appearance of variability are known, but the exact mechanism has not yet been determined. In this paper, we used next-generation sequencing and comparative genomics to study changes in the genomes of cucumber lines resulting from in vitro regeneration and somaclonal mutation in comparison to a reference, the highly inbred B10 line. The total number of obtained polymorphisms differed between the three somaclonal lines S1, S2 and S3, with 8369, 7591 and 44510, respectively. Polymorphisms occurred most frequently in non-coding regions and were mainly SNPs. High-impact changes accounted for 1Percent -3Percent of all polymorphisms and most often caused an open reading frame shift. Functional analysis of genes affected by high impact variants showed that they were related to transport, biosynthetic processes, nucleotide-containing compounds and cellular protein modification processes. The obtained results indicated significant factors affecting somaclonal variability and the appearance of changes in the genome, and demonstrated a lack of dependence between phenotype and the number of genomic polymorphisms.
650	1	4	_aCOMPARATIVE GENOMICS
650	1	4	_aCUCUMBER
650	1	4	_aIN VITRO REGENERATION
650	1	4	_aSNP
650	1	4	_aSMALL NUCLEOTIDE POLYMORPHISM
650	1	4	_aSOMACLONAL VARIATION
700	1	2	_aSkarzynska, A.
700	1	2	_aPawelkowicz, M.
700	1	2	_aPlader, W.
856	4	0	_uhttps://drive.google.com/file/d/18MQ2NjGgyHIdWqTYliD7Qf827PgpNlOx/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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