000			02188nam a2200313Ia 4500
003			MX-MdCICY
005			20250625162421.0
040			_cCICY
090			_aB-18915
245	1	0	_aIntegration of lipidomic and transcriptomic profiles reveals novel genes and regulatory mechanisms of Schizochytrium sp. in response to salt stress
490	0		_vBioresource Technology, 294, p.122231, 2019
520	3		_aIn this study, the effects of salt stress on the physiological, lipidomic and transcriptomic profiles of halophilic microalga Schizochytrium sp. were investigated. In general, Schizochytrium sp. could survive under high osmotic fermentation medium containing 30 g/L NaCl, and showed a significant increase in C14:0 percentage in total fatty acids. In lipidomic analysis, C14:0 was specifically enriched in phosphatidylcholine (PC), and membrane phospholipids participated in the salt stress response mostly. Specially, one novel signal lipid N-acylphosphatidylethanolamine (NAPE)(18:0/20:3/14:0)was upregulated significantly. Transcriptomic analysis revealed glycerol-3-phosphate acyltransferase (GPAT)and phospholipase ABHD3 (PLABDH3)were involved in C14:0 metabolism and NAPE biosynthesis. Signalling pathways they mediated were activated as evident by high expression level of Myristoyl-CoA: protein N-myristoyltransferase (NMT)and NAPE-hydrolyzing PLD (NAPE-PLD). This study gives us an insight in specific responses to salt stress in Schizochytrium sp. and provides a considerable proportion of novel genes that could commendably be used for engineering modification.
650	1	4	_aACYLTRANSFERAS
650	1	4	_aHALOPHILE
650	1	4	_aLIPIDOMICS
650	1	4	_aN-ACYLPHOSPHATIDYLETHANOLAMINE
650	1	4	_aSCHIZOCHYTRIUM SP.
650	1	4	_aTRANSCRIPTOMICS
700	1	2	_aJiang, J. Y.
700	1	2	_aZhu, S.
700	1	2	_aZhang, Y.
700	1	2	_aSun, X.
700	1	2	_aHu, X.
700	1	2	_aHuang, H.
700	1	2	_aRen, L. J.
856	4	0	_uhttps://drive.google.com/file/d/1AM-Y7UXgGrKF2SHHDDGJ5EzgeOAXpBKo/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c53058 _d53058