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| 003 | MX-MdCICY | ||
| 005 | 20250625162422.0 | ||
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| 090 | _aB-18945 | ||
| 245 | 1 | 0 | _aConstruction of an infectious clone of a plant RNA virus in a binary vector using one-step Gibson Assembly |
| 490 | 0 | _vJournal of virological method, 222, p.11-15, 2015 | |
| 520 | 3 | _aThe construction of full-length infectious clones of RNA viruses is often laborious due to the many cloning steps required and the DNA exclusion within the plasmid during Escherichia coli transformation. We demonstrate single-step cloning procedure of an infectious cDNA of the tomato blistering mosaic virus (ToBMV)using Gibson Assembly (GA), which drastically reduces the number of cloning steps. By agro-inoculation with the construct obtained by this procedure, ToBMV was recovered six days post-inoculation in Nicotiana benthamiana plants. The symptoms induced by the recovered virus were indistinguishable from those caused by the wild-type virus. We conclude that the GA is very useful method particularly to construct a full-length cDNA clone of a plant RNA virus in a binary vector. | |
| 650 | 1 | 4 | _aFULL-LENGTH CDNA CLONE |
| 650 | 1 | 4 | _aGIBSON ASSEMBLY |
| 650 | 1 | 4 | _aSEAMLESS CLONING |
| 650 | 1 | 4 | _aTYMOVIRUS |
| 700 | 1 | 2 | _aBlawid, R. |
| 700 | 1 | 2 | _aNagata, T. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1q8wEqKxx0QD65ZOfX60ve6WaKJzYOgYW/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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