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090			_aB-18946
245	1	0	_aConstruction of an agroinfectious clone of bean rugose mosaic virus using Gibson Assembly
490	0		_vVirus genes, 53(3), p.495-499, 2017
520	3		_aConstruction of agroinfectious viral clones usually requires many steps of cloning and sub-cloning and also a binary vector, which makes the process laborious, time-consuming, and frequently susceptible to some degree of plasmid instability. Nowadays, novel methods have been applied to the assembly of infectious viral clones, and here we have applied isothermal, single-step Gibson Assembly (GA)to construct an agroinfectious clone of Bean rugose mosaic virus (BRMV)using a small binary vector. The procedure has drastically reduced the cloning steps, and BRMV could be recovered from agroinfiltrated common bean twenty days after inoculation, indicating that the infectious clone could spread in the plant tissues and efficiently generate a systemic infection. The virus was also recovered from leaves of common bean and soybean cultivars mechanically inoculated with infectious clone two weeks after inoculation, confirming the efficiency of GA cloning procedure to produce the first BRMV agroinfectious clone to bean and soybean.
650	1	4	_aBEAN RUGOSE MOSAIC VIRUS
650	1	4	_aCOMOVIRUS
650	1	4	_aGIBSON ASSEMBLY
650	1	4	_aINFECTIOUS CLONE
650	1	4	_aISOTHERMAL REACTION
700	1	2	_aBijora, T.
700	1	2	_aBlawid, R.
700	1	2	_aCosta, D. K.
700	1	2	_aAragão, F. J.
700	1	2	_aSouto, E. R.
700	1	2	_aNagata, T.
856	4	0	_uhttps://drive.google.com/file/d/1ascGZx8yi-hXE3oZe0haLwnyZEmwdYCD/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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