000			01878nam a2200241Ia 4500
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005			20250625162422.0
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090			_aB-18977
245	1	0	_aDynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration
490	0		_vDevelopmental Cell, 57, p.1-17, 2022
520	3		_aShoot regeneration is mediated by the sequential action of two phytohormones, auxin and cytokinin. However, the chromatin regulatory landscapes underlying this dynamic response have not yet been studied. In this study, we jointly profiled chromatin accessibility, histone modifications, and transcriptomes to demonstrate that a high auxin/cytokinin ratio environment primes Arabidopsis shoot regeneration by increasing the accessibility of the gene loci associated with pluripotency and shoot fate determination. Cytokinin signaling not only triggers the commitment of the shoot progenitor at later stages but also allows chromatin to maintain shoot identity genes at the priming stage. Our analysis of transcriptional regulatory dynamics further identifies a catalog of regeneration cis-elements dedicated to cell fate transitions and uncovers important roles of BES1, MYC, IDD, and PIF transcription factors in shoot regeneration. Our results, thus, provide a comprehensive resource for studying cell reprogramming in plants and provide potential targets for improving future shoot regeneration efficiency.
700	1	2	_aWu, L. Y
700	1	2	_aShang, G. D
700	1	2	_aWang, F. X
700	1	2	_aGao, J
700	1	2	_aWan, M. C
700	1	2	_aXu, Z. G
700	1	2	_aWang, J. W.
856	4	0	_uhttps://drive.google.com/file/d/1OoCZm7l6_gbZpj-QkQRm66_MFKVtA8Nt/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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