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245	1	0	_aUltrahigh-Performance Liquid Chromatography-Mass Spectrometry Analysis of Carotenoid-Derived Hormones and Apocarotenoids in Plants
490	0		_vCurrent Protocols, 2(2), p.e375, 2022
520	3		_aCarotenoid oxidative cleavage products, apocarotenoids (APOs), are a class of important plant secondary metabolites, which include phytohormones abscisic acid (ABA)and strigolactones (SLs), and growth regulators and signaling molecules such as ?-cyclocitral, zaxinone, anchorene, ?-apo-11-carotenoids, and retinal. Qualitative and quantitative analysis of these bioactive compounds is crucial for understanding their metabolism and may also enable discovering further regulatory APOs. The state-of-the-art mass spectrometry (MS)technology has advanced the detection of plant APOs; however, it is still challenging to perform an accurate analysis of the low-level phytohormones ABA and SL and the structurally diverse APOs from complex plant matrices. Here, we describe ultrahigh-performance liquid chromatography-MS (UHPLC-MS)methods to determine carotenoid-derived hormones and APOs from plants by integrating ultrasound-assisted extraction and solid-phase extraction. These assays enable an accurate quantification of carotenoid-derived hormones and APOs from plant tissues by using an UHPLC hybrid quadrupole-Orbitrap mass spectrometer.
650	1	4	_aABSCISIC ACID
650	1	4	_aAPOCAROTENOIDS
650	1	4	_aRICE ROOT
650	1	4	_aSOLID-PHASE EXTRACTION
650	1	4	_aSTRIGOLACTONES
650	1	4	_aULTRASOUND-ASSISTED EXTRACTION
650	1	4	_aULTRAHIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
700	1	2	_aMi, J.
700	1	2	_aLiew, K. X.
700	1	2	_aAl-Babili, S.
856	4	0	_uhttps://drive.google.com/file/d/1iXfIAnefx2dJwbCOYnGtcRpKEsDw2dbh/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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