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| 003 | MX-MdCICY | ||
| 005 | 20250625162425.0 | ||
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| 090 | _aB-19121 | ||
| 245 | 1 | 0 | _aAlendronate crosslinked chitosan/polycaprolactone scaffold for bone defects repairing |
| 490 | 0 | _vInternational Journal of Biological Macromolecules, 204, p.441-456, 2022 | |
| 520 | 3 | _aHere, we evaluated osteogenic differentiation in vitro and new bone formation in vivo using an alendronate-loaded chitosan/polycaprolactone scaffold (CS/PCL)in rats with a critical-sized calvarial defect. Through the action of genipin, which has a crosslinking function, alendronate (AL)was anchored throughout the CS/PCL composite scaffold (CS/PCL@AL)to form an AL sustained release system. We demonstrated that CS/PCL@AL scaffolds significantly enhanced the osteogenic differentiation of ectomesenchymal stem cells (EMSCs)in vitro. Additionally, we explored the possible molecular mechanism of CS/PCL@AL scaffolds in the osteogenic differentiation of EMSCs. This composite scaffold exerted two positive effects on EMSC osteogenic differentiation: 1)the CS/PCL@AL scaffold enhanced EMSC osteogenic differentiation by upregulating bone morphogenetic protein 2, interleukin 10 and laminin expression; and 2)the CS/PCL@AL scaffold promoted the osteogenic differentiation of EMSCs by activating the yes-associated protein (YAP)signaling pathway. YAP and its downstream target transglutaminase are crucial mediators in the osteogenic differentiation of EMSCs. Finally, micro-computed tomography analyses and histology results suggested that the CS/PCL@AL scaffold exhibited a superior capacity to accelerate new and mature bone formation in skull bone defects in Sprague-Dawley rats. This simple and low-cost technology may represent a promising strategy to construct an efficient delivery system to repair bone defects. | |
| 650 | 1 | 4 | _aCHITOSAN |
| 650 | 1 | 4 | _aALENDRONATE |
| 650 | 1 | 4 | _aPCL |
| 700 | 1 | 2 | _aShi, W. |
| 700 | 1 | 2 | _aZhang, X. |
| 700 | 1 | 2 | _aBian, L. |
| 700 | 1 | 2 | _aDai, Y. |
| 700 | 1 | 2 | _aWang, Z. |
| 700 | 1 | 2 | _aZhou, Y. |
| 700 | 1 | 2 | _aLu, X. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1FP4nyZIckkMpLkP4rxq0Ptpr3BUXR90z/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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