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090 _aB-19123
245 1 0 _aPolymorphism and methylation patterns in Agave tequilana Weber var. 'Azul' plants propagated asexually by three different methods
490 0 _vPlant Science, 185, p.321-330, 2012
520 3 _aGenetic variation in three forms of asexually propagated Agave tequilana Weber var. 'Azul' plants namely offsets, bulbils and in vitro cultured individuals was studied by AFLP analysis. Low levels of variation were observed between mother plants and offsets and a higher level between mother plant and bulbils. Families obtained from commercial plantations showed lower levels of variation in comparison to families grown as ornamentals. No variation was observed between the original explant and four generations of in vitro cultured plants. Epigenetic variation was also studied by analyzing changes in methylation patterns between mother plants and offspring in each form of asexual reproduction. Offsets and bulbils showed an overall decrease in methylation whereas in vitro cultured plants showed patterns specific to each generation: Generations 1 and 4 showed overall demethylation whereas Generations 2 and 3 showed increased methylation. Analysis of ESTs associated with transposable elements revealed higher proportions of ESTs from Ty1-copia-like, Gypsy and CACTA transposable elements in cDNA libraries obtained from pluripotent tissue suggesting a possible correlation between methylation patterns, expression of transposable element associated genes and somaclonal variation.
650 1 4 _aAGAVE TEQUILANA
650 1 4 _aASEXUAL REPRODUCTION
650 1 4 _aIN VITRO CULTURE
650 1 4 _aAFLP
650 1 4 _aMSAP
650 1 4 _aTRANSPOSABLE ELEMENT ESTS
700 1 2 _aDíaz-Martínez, M.
700 1 2 _aNava-Cedillo, A.
700 1 2 _aGuzmán-López, J. A.
700 1 2 _aEscobar-Guzmán, R.
700 1 2 _aSimpson, J.
856 4 0 _uhttps://drive.google.com/file/d/1IBvoY8jHv9Kyd9R3taInWrou6Fecdjia/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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