000			02697nam a2200313Ia 4500
003			MX-MdCICY
005			20250625162425.0
040			_cCICY
090			_aB-19130
245	1	0	_aChromosome-level pepino genome provides insights into genome evolution and anthocyanin biosynthesis in Solanaceae
490	0		_vThe Plant Journal, doi: 10.1111/tpj.15728, 2022
520	3		_aPepino (Solanum muricatum, 2n = 2x = 24), a member of the Solanaceae family, is an important globally grown fruit. Herein, we report high-quality, chromosome-level pepino genomes. The 91.67 percent genome sequence is anchored to 12 chromosomes, with a total length of 1.20 Gb and scaffold N50 of 87.03 Mb. More than half the genome comprises repetitive sequences. In addition to the shared ancient whole-genome triplication (WGT)event in eudicots, an additional new WGT event was present in the pepino. Our findings suggest that pepinos experienced chromosome rearrangements, fusions, and gene loss after a WGT event. The large number of gene removals indicated the instability of Solanaceae genomes, providing opportunities for species divergence and natural selection. The paucity of disease-resistance genes (NBS)in pepino and eggplant has been explained by extensive loss and limited generation of genes after WGT events in Solanaceae. The outbreak of NBS genes was not synchronized in Solanaceae species, which occurred before the Solanaceae WGT event in pepino, tomato, and tobacco, whereas it was almost synchronized with WGT events in the other four Solanaceae species. Transcriptome and comparative genomic analyses revealed several key genes involved in anthocyanin biosynthesis. Although an extra WGT event occurred in Solanaceae, CHS genes related to anthocyanin biosynthesis in grapes were still significantly expanded compared with those in Solanaceae species. Proximal and tandem duplications contributed to the expansion of CHS genes. In conclusion, the pepino genome and annotation facilitate further research into important gene functions and comparative genomic analysis in Solanaceae.
650	1	4	_aSOLANACEAE
650	1	4	_aANTHOCYANIN BIOSYNTHESIS GENES
650	1	4	_aGENOME EVOLUTION
650	1	4	_aPEPINO GENOME
650	1	4	_aRESISTANCE GENES
650	1	4	_aWHOLE-GENOME TRIPLICATION
700	1	2	_aSong, X.
700	1	2	_aLiu, H.
700	1	2	_aShen, S.
700	1	2	_aHuang, Z.
700	1	2	_aYu, T.
700	1	2	_aLiu, Z.
700	1	2	_aDuan, W.
856	4	0	_uhttps://drive.google.com/file/d/1WMzcjJxgWC6CmVrIjY9K8D3Dkar9Jkc1/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c53269 _d53269