000 02178nam a2200193Ia 4500
003 MX-MdCICY
005 20250625162428.0
040 _cCICY
090 _aB-19293
245 1 0 _aChanges in fatty acid composition during development of tissues of coconut (Cocos nucifera L.)embryos in the intact nut and in vitro
490 0 _vJournal of Experimental botany, 52(358), p.933-942, 2001
520 3 _aIntact coconuts were germinated in situ and compared with excised zygotic embryos germinated in vitro. The growth of the embryonic tissue and their fatty acid compositions were measured. Haustoria, plumules and radicles of coconuts germinated in situ grew continuously and proportionately throughout the 120 d experiment with haustauria increasing to 45 g x nut(-1)and weighing 4-5-fold more than the other two tissues. The plumules and radicles of the seedlings cultured in vitro also grew continuously but the haustoria grew sporadically between 15 d and 75 d in culture and, at 250 mg x nut(-1)after 75 d, were smaller than the other two tissues. All the tissues of the nuts grown in situ contained significant amounts of lauric acid, the acid characteristic of coconut oil, as well as longer chain saturated and unsaturated fatty acids. The content of medium and long chain fatty acids increased in all growing tissues as the experiment proceeded, especially the haustorium which contained 24-35 percent of its fatty acid as lauric acid; the fat content of solid endosperm reduced during this period. Seedlings grown in vitro, on the other hand, failed to accumulate lauric acid in any of their tissues (haustorium contained 6-11 percent of its fatty acid as lauric acid). The results may have implications for the design of growth media for growing zygotic and somatic cultures of coconut and may provide a marker for successful germination.
700 1 2 _aLópez-Villalobos, A.
700 1 2 _aDodds, P. F.
700 1 2 _aHornung, R.
856 4 0 _uhttps://drive.google.com/file/d/1oVERmbWIGj5b7HWa2qKGJU6CXnljBikt/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 250602s9999 xx |||||s2 |||| ||und|d
999 _c53430
_d53430