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245 1 0 _aVacuum liquid chromatographic isolation and characterization of beta-sitosterol from the bark of Sarcocephalus latifolius (Smith Bruce).
490 0 _vJournal of Chemistry and Chemical Sciences, 5(9), p.480-488, 2015
520 3 _aThe methanol fraction of the stem bark of Sarcocephalus Latifolius (Smith Bruce)was subjected to vacuum liquid chromatography (VLC)isolation method using an isocratic solvent of petroleum ether: ethyl acetate at a ratio of 1:9 by collecting 20ml each and 45 fractions were collected. Based on their Thin Layer Chromatographic (TLC)analysis, fraction 6, 7, 8 and 9 were combined to give SB-C which was further subjected to VLC and eluted isocratically using petroleum ether: ethyl acetate (1:9)to obtain SB-C-1 to SB-C-8 (8 Fraction). Fraction 5 shows a prominent single yellow spot that was cleaned further by Preparative Thin Layer Chromatography (PTLC)to obtain SB-C1. The structure of the compound was established by spectroscopic studies as well as by comparison with existing data. Based on these SB-C1 was proposed to be _-Sitosterol.
650 1 4 _aSARCOCEPHALUS
650 1 4 _aISOLATION
650 1 4 _aMETHANOL
650 1 4 _aCHROMATOGRAPHY
650 1 4 _aSPECTROSCOPIC
650 1 4 _a_-SITOSTEROL
700 1 2 _aYinusa, I.
700 1 2 _aGeorge, N. I.
700 1 2 _aAyo, R. G.
700 1 2 _aRufai, Y.
856 4 0 _uhttps://drive.google.com/file/d/1Z0iKERIYVrIZ8o-RQFvn3NKd0WBQoASq/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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