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003			MX-MdCICY
005			20250625162429.0
040			_cCICY
090			_aB-19389
245	1	0	_aPhysiological, epigenetic, and proteomic responses in Pfaffia glomerata growth in vitro under salt stress and 5-azacytidine
490	0		_vProtoplasma, 10.1007/s00709-022-01789-4, 2022
520	3		_aPlants adjust their complex molecular, biochemical, and metabolic processes to overcome salt stress. Here, we investigated the proteomic and epigenetic alterations involved in the morphophysiological responses of Pfaffia glomerata, a medicinal plant, to salt stress and the demethylating agent 5-azacytidine (5-azaC). Moreover, we investigated how these changes affected the biosynthesis of 20-hydroxyecdysone (20-E), a pharmacologically important specialized metabolite. Plants were cultivated in vitro for 40 days in Murashige and Skoog medium supplemented with NaCl (50 mM), 5-azaC (25 ?M), and NaCl + 5-azaC. Compared with the control (medium only), the treatments reduced growth, photosynthetic rates, and photosynthetic pigment content, with increase in sucrose, total amino acids, and proline contents, but a reduction in starch and protein. Comparative proteomic analysis revealed 282 common differentially accumulated proteins involved in 87 metabolic pathways, most of them related to amino acid and carbohydrate metabolism, and specialized metabolism. 5-azaC and NaCl + 5-azaC lowered global DNA methylation levels and 20-E content, suggesting that 20-E biosynthesis may be regulated by epigenetic mechanisms. Moreover, downregulation of a key protein in jasmonate biosynthesis indicates the fundamental role of this hormone in the 20-E biosynthesis. Taken together, our results highlight possible regulatory proteins and epigenetic changes related to salt stress tolerance and 20-E biosynthesis in P. glomerata, paving the way for future studies of the mechanisms involved in this regulation.
650	1	4	_aABIOTIC STRESS
650	1	4	_aDNA METHYLATION
650	1	4	_aMEDICINAL PLANT
650	1	4	_aMETHYLTRANSFERASE INHIBITOR
650	1	4	_aPHYTOECDYSTEROIDS
650	1	4	_aPROTEOMICS
700	1	2	_aFortini, E. A.
700	1	2	_aBatista, D. S.
700	1	2	_aFelipe, S. H. S.
700	1	2	_aSilva, T. D.
700	1	2	_aCorreia, L. N. F.
700	1	2	_aFarias, L. M.
700	1	2	_aOtoni, W. C.
856	4	0	_uhttps://drive.google.com/file/d/1ZoKuZND9H-7fItq5j1J62FHq5eSbqB6z/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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