| 000 | 02334nam a2200313Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162431.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-19459 | ||
| 245 | 1 | 0 | _amiR156 regulates somatic embryogenesis by modulating starch accumulation in citrus |
| 490 | 0 | _vJournal of Experimental Botany, https://doi.org/10.1093/jxb/erac248, 2022 | |
| 520 | 3 | _aSomatic embryogenesis (SE)is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remains unclear. In this study, we found that the transcription factors (TFs)CsAGL15 and CsFUS3 bind to the CsMIR156A promoter and activate its expression. Suppression of csi-miR156a function leads to up-regulation of four target genes CsSPLs and reduction of SE efficiency. In the STTM-miR156a overexpressed callus (MIM156), the number of amyloplasts and starch content were significantly reduced, and genes involved in starch synthesis and transport were down-regulated; csi-miR172d was down-regulated, whereas the target genes CsTOE1.1 and CsTOE1.2, which inhibit the expression of starch biosynthesis genes, were up-regulated. In our working model, CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPLs and further regulates csi-miR172d and CsTOEs, thus to alter starch accumulation level in callus cells and regulate SE in citrus. This study elucidates the pathway of miR156-SPLs and miR172-TOEs mediated regulation of SE, and provides clues to enhancing SE capability in citrus. | |
| 650 | 1 | 4 | _aCALLUS |
| 650 | 1 | 4 | _aCITRUS |
| 650 | 1 | 4 | _aMIR156 |
| 650 | 1 | 4 | _aMIR172 |
| 650 | 1 | 4 | _aSOMATIC EMBRYOGENESIS |
| 650 | 1 | 4 | _aSTARCH |
| 700 | 1 | 2 | _aFeng, M. Q. |
| 700 | 1 | 2 | _aLu, M. D. |
| 700 | 1 | 2 | _aLong, J. M. |
| 700 | 1 | 2 | _aYin, Z. P. |
| 700 | 1 | 2 | _aJiang, N. |
| 700 | 1 | 2 | _aWang, P. B. |
| 700 | 1 | 2 | _aWu, X. M. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/15iuq3Xsgdk6bKK37yvs-V_om0uwm8TMj/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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