| 000 | 02252nam a2200265Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625162431.0 | ||
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| 090 | _aB-19475 | ||
| 245 | 1 | 0 | _aDevelopment of polyclonal antibodies-based serological methods and a DIG-labelled DNA probe-based molecular method for detection of the Vicia cryptic virus-M in field plants |
| 490 | 0 | _vJournal of Virological Methods, 299, p.114331, 2022 | |
| 520 | 3 | _aVicia cryptic virus M (VCV-M), a member of the genus Amalgavirus of the family Amalgaviridae, was first identified in 2009 in a Vicia faba Linn. planting in Hangzhou, Zhejiang Province, China. However, there has been no further research on the biological features of VCV-M to date and the viral particles and coat protein (CP)have not been identified. The putative CP of VCV-M was predicted from the viral genomic RNA. In this study, a recombinant version of the putative CP of VCV-M (His-CPVCV-M)was produced and used to prepare a polyclonal antiserum against the His-CPVCV-M. Using this antiserum, a Western blot, an immuno-dot-blot and an enzyme-linked immunosorbent assay were developed for testing field samples of V. faba for the presence of VCV-M. Additionally, a digoxigenin (DIG)-labelled DNA probe-based Northern blot assay was established for VCV-M genome detection in field samples. The results showed that both the serological and nucleic acid assays could accurately and sensitively detect VCV-M in V. faba. This research represented the first confirmed expression of the putative CP of VCV-M in infected V. faba tissues. The serological and nucleic acid assays provided two complementary methods for VCV-M detection which could contribute to seed quality control and production increases of V. faba crops. | |
| 650 | 1 | 4 | _aDIG-LABELLED PROBE |
| 650 | 1 | 4 | _aPOLYCLONAL ANTISERUM |
| 650 | 1 | 4 | _aVICIA CRYPTIC VIRUS M. |
| 700 | 1 | 2 | _aZhang, K. |
| 700 | 1 | 2 | _aZhuang, X. |
| 700 | 1 | 2 | _aXu, H. |
| 700 | 1 | 2 | _aGan, H. |
| 700 | 1 | 2 | _aHe, Z. |
| 700 | 1 | 2 | _aChen, J. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1bRfBtZf93PIM6N0rEmC3PPq1-3cH5d4f/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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