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040			_cCICY
090			_aB-19548
245	1	0	_aBiosynthesis of the sesquiterpene germacrene D in Solidago canadensis: 13C and 2H labeling studies
490	0		_vPhytoChemistry, 60(1), p.13-20, 2002
520	3		_aThe biogenetic origin of the isoprenoid building blocks of the sesquiterpene germacrene D was studied in Solidago canadensis. Feeding experiments were carried out with 1-[5,5-D(2)]deoxy-D-xylulose-5-phosphate (D(2)-DOXP), [5-13C]mevalonolactone (13C-MVL)and [1-13C]-D-glucose. The hydrodistillate of a cut shoot fed with D(2)-DOXP was investigated by enantio-MDGC-MS and the volatile fraction of a shoot supplied with 13C-MVL was examined by GC-C-IRMS. The incorporation of [1-13C]-D-glucose was analyzed by quantitative 13C NMR spectroscopy after isolation of germacrene D from the essential oil. Our labeling studies revealed that the biosynthesis of the C-15 skeleton of sesquiterpene germacrene D in Solidago canadensis proceeds predominantly via the methylerythritol phosphate pathway.
650	1	4	_aSOLIDAGO CANADENSIS
650	1	4	_aASTERACEAE
650	1	4	_aBIOSYNTHESIS
650	1	4	_aMETHYLERYTHRITOL PHOSPHATE PATHWAY
650	1	4	_aSESQUITERPENES
650	1	4	_aGERMACRENE D
650	1	4	_aLABELING STUDIES
700	1	2	_aSteliopoulos, P.
700	1	2	_aWüst, M.
700	1	2	_aAdam, K. P.
700	1	2	_aMosandl, A.
856	4	0	_uhttps://drive.google.com/file/d/1klXLYLlxt5Zujg9B59xfVqxJLv5HMvEv/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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