000			02621nam a2200301Ia 4500
003			MX-MdCICY
005			20250625162434.0
040			_cCICY
090			_aB-19650
245	1	0	_aPestalotiopsis mangiferae isolated from cocoa leaves and concomitant tannase and gallic acid production
490	0		_vFungal Biology, 126, p.471-479, 2022
520	3		_aThe enzyme tannase is of great industrial and biotechnological importance for the hydrolysis of vegetable tannins, reducing their undesirable effects and generating products for a wide range of processes. Thus, the search for new microorganisms that permit more stable tannase production is of considerable importance. A strain of P. mangiferae isolated from cocoa leaves was selected and investigated for its capacity to produce tannase enzymes and gallic acid through submerged fermentation. The assessment of the variables affecting tannase production by P. mangiferae showed that tannic acid, ammonium nitrate and temperature were the most significant (8.4 U/mL). The variables were analyzed using Response Surface Methodology - RSM (Box-Behnken design), with the best conditions for tannase production being: 1.9 percent carbon source, 1 percent nitrogen source and temperature of 23 °C. Tannase activity doubled (16.9 U/mL)after the optimization process when compared to the initial fermentation. A pH of 7.0 was optimal for the tannase and it presented stability above 80 percent with pH between 4.0 and 7.0 after 2h of incubation. The optimal temperature was 30 °C and activity remained at above 80 percent at 40-60 °C after 1 h. Production of gallic acid was achieved with 1 percent tannic acid (0.9 mg/mL)and P. mangiferae had not used up the gallic acid produced by tannic acid hydrolysis after 144 h of fermentation. A 5 percent tannic acid concentration was the best for gallic acid production (1.6 mg/mL). These results demonstrate P. mangiferae's potential for tannase and gallic acid production for biotechnological applications.
650	1	4	_aRESPONSE SURFACE METHODOLOGY
650	1	4	_aBIOCONVERSION
650	1	4	_aCOCOA
650	1	4	_aFILAMENTOUS FUNGI
650	1	4	_aENZYME
700	1	2	_aDe Lima, C. S.
700	1	2	_aKoelher, B. T. A.
700	1	2	_aDa Silva, E. G. P.
700	1	2	_aGóes-Neto, A.
700	1	2	_aRezende, R. P.
700	1	2	_aUetanabaro, A. P. T.
700	1	2	_aDa Costa, A. M.
856	4	0	_uhttps://drive.google.com/file/d/1YIVeZTXpSECUyBaKXwXWtPJ5_lHDkFUS/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c53773 _d53773